IHC-plus(tm) RETN / Resistin Antibody

LifeSpan BioSciences

IHC-plus(tm) RETN / Resistin Antibody

23

Immunohistochemistry: LS-B7412 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with biotinylated antigen affinity purified anti-human Resistin (LS-C104464) as the detection antibody at a concentration of at least 0.5-1 ug/ml. Western Blot: To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.. ELISA, IHC, IHC-P (10 µg/ml), WB (0.2 - 0.4 µg/ml) Immunohistochemistry: LS-B7412 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with biotinylated antigen affinity purified anti-human Resistin (LS-C104464) as the detection antibody at a concentration of at least 0.5-1 ug/ml. Western Blot: To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

Unconjugated

RETN

resistin

ADSF, FIZZ3, RENT, RETN1, RSTN, XCP1, resistin, C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1, adipose tissue-specific secretory factor, c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, cysteine-rich secreted protein A12-alpha-like 2, cysteine-rich secreted protein FIZZ3, found in inflammatory zone 3, resistin delta2

Mouse

IgG2b

Human

-20°C,2°C to 8°C,RT

41116161