IHC-plus(tm) RNF25 Antibody

LifeSpan BioSciences

IHC-plus(tm) RNF25 Antibody

23

The antibody also recognizes mouse RNF25 protein. Reactivity against homologues from other sources has not been determined.

Immunohistochemistry: LS-B418 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B418 was determined to be 5 ug/ml.. ELISA (1:10000 - 1:40000), IHC, IHC-P (5 µg/ml), IP, WB (1:200 - 1:2000) Immunohistochemistry: LS-B418 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B418 was determined to be 5 ug/ml.

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

RNF25

ring finger protein 25

AO7; E3 ubiquitin-protein ligase RNF25; ring finger protein AO7; RING-type E3 ubiquitin transferase RNF25

Rabbit

IgG

Human, Mouse

2°C to 8°C or -20°C

12352203