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ProductsBack/IHC-plus(tm) SCN2A / Nav1.2 Antibody (aa983-1032)

IHC-plus(tm) SCN2A / Nav1.2 Antibody (aa983-1032)

LS-B12560
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSCN2A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SCN2A / Nav1.2 Antibody (aa983-1032)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 227 kDa
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:50), Peptide-ELISA (1:10000) Western Blot: Predicted MW 227 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6326
  • Target name
    SCN2A
  • Target description
    sodium voltage-gated channel alpha subunit 2
  • Target synonyms
    BFIC3, BFIS3, BFNIS, DEE11, EA9, EIEE11, HBA, HBSCI, HBSCII, NAC2, Na(v)1.2, Nav1.2, SCN2A1, SCN2A2, sodium channel protein type 2 subunit alpha, sodium channel protein brain II subunit alpha, sodium channel protein type II subunit alpha, sodium channel protein, brain type 2 alpha subunit, sodium channel, voltage-gated, type II, alpha 1 polypeptide, sodium channel, voltage-gated, type II, alpha 2 polypeptide, sodium channel, voltage-gated, type II, alpha subunit, voltage-gated sodium channel subtype II, voltage-gated sodium channel subunit alpha Nav1.2, voltage-gated sodium channel type II alpha subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCN2A using anti-SCN2A antibody (A01735-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCN2A antigen affinity purified polyclonal antibody (Catalog # A01735-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCN2A at approximately 320KD. The expected band size for SCN2A is at 320KD.
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