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ProductsBack/IHC-plus(tm) SLC39A10 / ZIP10 Antibody

IHC-plus(tm) SLC39A10 / ZIP10 Antibody

LS-B5357
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC39A10
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SLC39A10 / ZIP10 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    ZIP10 antibody can be used for detection of ZIP10 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:. ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) ZIP10 antibody can be used for detection of ZIP10 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57181
  • Target name
    SLC39A10
  • Target description
    solute carrier family 39 member 10
  • Target synonyms
    LZT-Hs2, ZIP10, zinc transporter ZIP10, ZIP-10, ZRT/IRT-like protein 10, solute carrier family 39 (metal ion transporter), member 10, solute carrier family 39 (zinc transporter), member 10, zinc transporter 10, zrt- and Irt-like protein 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC39A10 using anti-SLC39A10 antibody (A09043-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC39A10 antigen affinity purified polyclonal antibody (Catalog # A09043-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC39A10 at approximately 94 kDa. The expected band size for SLC39A10 is at 94 kDa.
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