IHC-plus(tm) SLC9A3R1 / NHERF1 / EBP50 Antibody
LS-B2196
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetNHERF1
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) SLC9A3R1 / NHERF1 / EBP50 Antibody
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B2196 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2196 was determined to be 5 ug/ml.. IHC, IHC-P (5 µg/ml), IP, WB (1 µg/ml) Immunohistochemistry: LS-B2196 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2196 was determined to be 5 ug/ml.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID9368
- Target nameNHERF1
- Target descriptionNHERF family PDZ scaffold protein 1
- Target synonymsEBP50, NHE-RF, NHERF, NHERF-1, NPHLOP2, SLC9A3R1, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Na(+)/H(+) exchange regulatory cofactor 1, Na+/H+ exchange regulatory co-factor, SLC9A3 regulator 1, ezrin-radixin-moesin binding phosphoprotein-50, regulatory cofactor of Na(+)/H(+) exchanger, solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
- HostRabbit
- IsotypeIgG
- ReactivityHuman, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC41116161




![NHERF1 antibody detects NHERF1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NHERF1 stained by NHERF1 antibody (GTX134431) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX134431/GTX134431_43614_20191125_ICC_IF_R_w_23060620_206.webp)