IHC-plus(tm) SMAD3 Antibody (aa100-150)

LifeSpan BioSciences

IHC-plus(tm) SMAD3 Antibody (aa100-150)

23

Immunohistochemistry: LS-B2064 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2064 was determined to be 10 ug/ml.. IHC, IHC-P (10 µg/ml), WB (0.5 - 2 µg/ml) Immunohistochemistry: LS-B2064 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2064 was determined to be 10 ug/ml.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

SMAD3

SMAD family member 3

HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3, mothers against decapentaplegic homolog 3, MAD homolog 3, MAD, mothers against decapentaplegic homolog 3, SMA- and MAD-related protein 3, SMAD, mothers against DPP homolog 3, mad homolog JV15-2, mad protein homolog, mothers against DPP homolog 3

Rabbit

Bovine, Canine, Human, Monkey, Mouse, Porcine, Primate, Rat

-20°C,2°C to 8°C

41116161