IHC-plus(tm) SMAD6 Antibody (aa85-99)

LifeSpan BioSciences

IHC-plus(tm) SMAD6 Antibody (aa85-99)

23

Immunohistochemistry: LS-B2065 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2065 was determined to be 5 ug/ml.. IHC, IHC-P (5 µg/ml), WB (0.5 - 2 µg/ml) Immunohistochemistry: LS-B2065 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2065 was determined to be 5 ug/ml.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

SMAD6

SMAD family member 6

AOVD2, HsT17432, MADH6, MADH7, mothers against decapentaplegic homolog 6, MAD homolog 6, SMAD, mothers against DPP homolog 6, mothers against decapentaplegic, drosophila, homolog of, 6

Rabbit

IgG

Human, Porcine

-20°C,2°C to 8°C

41116161