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ProductsBack/IHC-plus(tm) SP1 Antibody (aa735-785)

IHC-plus(tm) SP1 Antibody (aa735-785)

LS-B14621
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SP1 Antibody (aa735-785)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:1000 - 1:5000, 2.5 µg/ml), IP (2 - 10 µg/mg lysate), WB (1:2000 - 1:10000) IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6667
  • Target name
    SP1
  • Target description
    Sp1 transcription factor
  • Target synonyms
    transcription factor Sp1, specificity protein 1
  • Host
    Goat
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of SP1 using anti-SP1 antibody (A00110-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP1 antigen affinity purified polyclonal antibody (Catalog # A00110-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP1 at approximately 90 kDa. The expected band size for SP1 is at 81 kDa.
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