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ProductsBack/IHC-plus(tm) SSR1 Antibody (clone 1C11)

IHC-plus(tm) SSR1 Antibody (clone 1C11)

LS-B6153
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSSR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SSR1 Antibody (clone 1C11)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.. ELISA, IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000) Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1C11
  • Conjugate
    Unconjugated
  • Gene ID6745
  • Target name
    SSR1
  • Target description
    signal sequence receptor subunit 1
  • Target synonyms
    TRAPA, translocon-associated protein subunit alpha, SSR alpha subunit, SSR-alpha, TRAP alpha, signal sequence receptor subunit alpha, signal sequence receptor, alpha, translocon-associated protein alpha subunit
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRAP Alpha/TRAPA/SSR1 using anti-TRAP Alpha/TRAPA/SSR1 antibody (A06993-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human U937 whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: rat stomach tissue lysates, Lane 11: rat PC-12 whole cell lysates, Lane 12: mouse liver tissue lysates, Lane 13: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAP Alpha/TRAPA/SSR1 antigen affinity purified polyclonal antibody (Catalog # A06993-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAP Alpha/TRAPA/SSR1 at approximately 36 kDa. The expected band size for TRAP Alpha/TRAPA/SSR1 is at 32 kDa.
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