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IHC-plus(tm) TAAR6 Antibody (Cytoplasmic Domain)

LS-A2312
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTAAR6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TAAR6 Antibody (Cytoplasmic Domain)
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (3 - 7 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID319100
  • Target name
    TAAR6
  • Target description
    trace amine associated receptor 6
  • Target synonyms
    TA4; TAR4; taR-4; TAR6; taR-6; trace amine receptor 4; trace amine receptor 6; trace amine-associated receptor 6; TRAR4
  • Host
    Rabbit
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TARC/CCL17/TAAR6 using anti-TARC/CCL17/TAAR6 antibody (A10349-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TARC/CCL17/TAAR6 antigen affinity purified polyclonal antibody (Catalog # A10349-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TARC/CCL17/TAAR6 at approximately 38 kDa. The expected band size for TARC/CCL17/TAAR6 is at 38 kDa.
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