More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IHC-plus(tm) TEK / TIE2 Antibody

IHC-plus(tm) TEK / TIE2 Antibody

LS-B14782
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTEK
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TEK / TIE2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 109kDa/121kDa/125kDa, while the observed MW by Western blot was 126kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000) The predicted MW is 109kDa/121kDa/125kDa, while the observed MW by Western blot was 126kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.39 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7010
  • Target name
    TEK
  • Target description
    TEK receptor tyrosine kinase
  • Target synonyms
    CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1, angiopoietin-1 receptor, TEK tyrosine kinase, endothelial, endothelial tyrosine kinase, tunica interna endothelial cell kinase, tyrosine kinase with Ig and EGF homology domains-2, tyrosine-protein kinase receptor TEK, tyrosine-protein kinase receptor TIE-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-TEK Antibody144-07222
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetTEK
  • SizePrice
Product group Antibodies
Biosynth
TIE2 antibody70R-TR005
  • SizePrice
Figure 1. Western blot analysis of TEK using anti-TEK antibody (A01274-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEK antigen affinity purified polyclonal antibody (Catalog # A01274-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEK at approximately 160KD. The expected band size for TEK is at 126KD.
Product group Antibodies
Boster Bio
Anti-TIE2/TEK Antibody Picoband(r)A01274-1-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTEK
  • SizePrice
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TIE2 antibody (GTX107505) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
TIE2 antibodyGTX107505
ApplicationsWestern Blot
ReactivityHuman
TargetTEK
  • SizePrice
Product group Antibodies
Assay Genie
TEK AntibodyCAB0743
ReactivityHuman
TargetTEK
  • SizePrice
Product group Antibodies
Biomatik
TEK Tyrosine Kinase, Endothelial (Tie2) Polyclonal AntibodyCAU28529
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetTEK
  • SizePrice
HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with _x000D_ TIE2/CD202b Polyclonal Antibody(bs-1300R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
TIE2 Polyclonal AntibodyBS-1300R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Equine, Human, Mouse, Porcine, Rat
TargetTEK
  • SizePrice
anti-TIE-2 (human), mAb (tek2)
Product group Antibodies
AdipoGen Life Sciences
anti-TIE-2 (human), mAb (tek2)AG-20T-0102
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetTEK
  • SizePrice
Western Blot analysis of VEC cells using Phospho-Tie-2 (Y992) Polyclonal Antibody
Product group Antibodies
Cusabio
Phospho-TEK (Y992) AntibodyCSB-PA006341
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetTEK
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TEK / TIE2 AntibodyLS-C402823
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetTEK
  • SizePrice