IHC-plus(tm) TF / Transferrin Antibody

LifeSpan BioSciences

IHC-plus(tm) TF / Transferrin Antibody

23

Immunohistochemistry: LS-B2447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2447 was determined to be 5 ug/ml. ELISA: 1:40000-1:160000 (Coat with 1 ug transferrin in a standard sandwich ELISA using peroxidase conjugated rabbit IgG and ABTS as a substrate for 30 min at RT).. ELISA (1:40000 - 1:160000), IHC, IHC-P (5 µg/ml), IP, WB Immunohistochemistry: LS-B2447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2447 was determined to be 5 ug/ml. ELISA: 1:40000-1:160000 (Coat with 1 ug transferrin in a standard sandwich ELISA using peroxidase conjugated rabbit IgG and ABTS as a substrate for 30 min at RT).

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

10 mg/ml

Unconjugated

TF

transferrin

HEL-S-71p, PRO1557, PRO2086, TFQTL1, serotransferrin, beta-1 metal-binding globulin, epididymis secretory sperm binding protein Li 71p, siderophilin

Rabbit

IgG

Human

2°C to 8°C or -20°C

41116161