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ProductsBack/IHC-plus(tm) TM9SF2 Antibody (aa111-140)

IHC-plus(tm) TM9SF2 Antibody (aa111-140)

LS-B12359
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTM9SF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TM9SF2 Antibody (aa111-140)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9375
  • Target name
    TM9SF2
  • Target description
    transmembrane 9 superfamily member 2
  • Target synonyms
    Lnc-PCIR, P76, transmembrane 9 superfamily member 2, 76 kDa membrane protein, LncRNA Positively Correlated with Inflammatory Responses, dinucleotide oxidase disulfide thiol exchanger 3 superfamily member 2, epididymis secretory sperm binding protein, transmembrane protein 9 superfamily member 2
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TM9SF2 using anti-TM9SF2 antibody (A13431). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM9SF2 antigen affinity purified polyclonal antibody (Catalog # A13431) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TM9SF2 at approximately 76 kDa. The expected band size for TM9SF2 is at 76 kDa.
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