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ProductsBack/IHC-plus(tm) TNFSF13 / APRIL Antibody

IHC-plus(tm) TNFSF13 / APRIL Antibody

LS-B3193
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTNFSF13
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TNFSF13 / APRIL Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    APRIL antibody can be used for detection of APRIL by Western blot at 1 - 4 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 27 kDa Observed: 27 kDa. ELISA, IHC, IHC-P (10 µg/ml), WB APRIL antibody can be used for detection of APRIL by Western blot at 1 - 4 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 27 kDa Observed: 27 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8741
  • Target name
    TNFSF13
  • Target description
    TNF superfamily member 13
  • Target synonyms
    a proliferation-inducing ligand; APRIL; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNLG7B; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand 7B; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; UNQ383/PRO715; ZTNF2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APRIL/TNFSF13 using anti-APRIL/TNFSF13 antibody (A02417-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APRIL/TNFSF13 antigen affinity purified polyclonal antibody (Catalog # A02417-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APRIL/TNFSF13 at approximately 32 kDa. The expected band size for APRIL/TNFSF13 is at 27 kDa.
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