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Western Blot analysis of COLO205 cells using APRIL Polyclonal Antibody
Western Blot analysis of COLO205 cells using APRIL Polyclonal Antibody
Western Blot analysis of COLO205 cells using APRIL Polyclonal Antibody

TNFSF13 Antibody

CSB-PA000909
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTNFSF13
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Overview

  • Supplier
    Cusabio
  • Product Name
    TNFSF13 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8741
  • Target name
    TNFSF13
  • Target description
    TNF superfamily member 13
  • Target synonyms
    APRIL, CD256, TALL-2, TALL2, TNLG7B, TRDL-1, UNQ383/PRO715, ZTNF2, tumor necrosis factor ligand superfamily member 13, TNF- and APOL-related leukocyte expressed ligand 2, a proliferation-inducing ligand, tumor necrosis factor (ligand) superfamily, member 13, tumor necrosis factor ligand 7B, tumor necrosis factor superfamily member 13, tumor necrosis factor-like protein ZTNF2, tumor necrosis factor-related death ligand-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75888
  • Protein Name
    Tumor necrosis factor ligand superfamily member 13
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APRIL/TNFSF13 using anti-APRIL/TNFSF13 antibody (A02417-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APRIL/TNFSF13 antigen affinity purified polyclonal antibody (Catalog # A02417-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APRIL/TNFSF13 at approximately 32 kDa. The expected band size for APRIL/TNFSF13 is at 27 kDa.
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