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ProductsBack/IHC-plus(tm) YES1 / c-Yes Antibody

IHC-plus(tm) YES1 / c-Yes Antibody

LS-B14851
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetYES1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) YES1 / c-Yes Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 60kDa, while the observed MW by Western blot was 61kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:50 - 1:100), IHC-P (1:200), WB (1:500 - 1:1000) The predicted MW is 60kDa, while the observed MW by Western blot was 61kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7525
  • Target name
    YES1
  • Target description
    YES proto-oncogene 1, Src family tyrosine kinase
  • Target synonyms
    HsT441, P61-YES, Yes, c-yes, tyrosine-protein kinase Yes, YES1 proto-oncogene, Src family tyrosine kinase, Yamaguchi sarcoma oncogene, cellular yes-1 protein, proto-oncogene c-Yes, proto-oncogene tyrosine-protein kinase YES, v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of YES1 using anti-YES1 antibody (PB9466). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YES1 antigen affinity purified polyclonal antibody (Catalog # PB9466) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YES1 at approximately 61 kDa. The expected band size for YES1 is at 61 kDa.
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