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ProductsBack/IKBKE / IKKI / IKKE Antibody (aa470-498)

IKBKE / IKKI / IKKE Antibody (aa470-498)

LS-C329667
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIKBKE
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IKBKE / IKKI / IKKE Antibody (aa470-498)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:1000), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9641
  • Target name
    IKBKE
  • Target description
    inhibitor of nuclear factor kappa B kinase subunit epsilon
  • Target synonyms
    I-kappa-B kinase epsilon; IKKE; IKK-E; IKK-epsilon; IKKI; IKK-i; IKK-related kinase epsilon; inducible I kappa-B kinase; inducible IkappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; inhibitor of nuclear factor kappa-B kinase subunit epsilon
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IKKi/IKKe/IKBKE using anti-IKKi/IKKe/IKBKE antibody (A01816-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKKi/IKKe/IKBKE antigen affinity purified polyclonal antibody (Catalog # A01816-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKKi/IKKe/IKBKE at approximately 80KD. The expected band size for IKKi/IKKe/IKBKE is at 80KD.
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