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ProductsBack/IL-10 Antibody (clone JES5-16E3, FITC)

IL-10 Antibody (clone JES5-16E3, FITC)

LS-C810819
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetIL10
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IL-10 Antibody (clone JES5-16E3, FITC)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results or refer to the dilution we used in the experiment.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results or refer to the dilution we used in the experiment.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    JES5-16E3
  • Concentration
    0.5 mg/ml
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID3586
  • Target name
    IL10
  • Target description
    interleukin 10
  • Target synonyms
    CSIF, GVHDS, IL-10, IL10A, TGIF, interleukin-10, T-cell growth inhibitory factor, cytokine synthesis inhibitory factor
  • Host
    Rat
  • Isotype
    IgG2b
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (A00021-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysate, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse SP20 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
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