IL-11: IL-11RA[Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

BPS Bioscience

IL-11: IL-11RA[Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

23

Research Use Only

Biotin

Interleukin-11

The IL-11:IL-11RA [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between IL-11RA (interleukin 11 receptor alpha subunit) and IL-11 for screening and profiling applications. This kit comes with enough purified IL-11 (amino acids 22-199) and biotinylated IL-11RA (amino acids 24-370), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. IL-11:IL-11RA [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with IL-11 protein. After coating and blocking, biotinylated IL-11RA is added in an optimized assay buffer. Next, unbound biotinylated IL-11RA is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL-11 binding to IL-11RA.Need us to run inhibitor screens or profile your compounds against IL-11:IL-11RA[Biotin]? Check out our Immunotherapy Biochemical Screening Services.

41116100

Human