Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-12 alpha Polyclonal Antibody, Unconjugated (bs-0767R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-12 alpha Polyclonal Antibody, Unconjugated (bs-0767R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
IL12A Polyclonal Antibody
BS-0767R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetIl12a
Overview
- SupplierBioss
- Product NameIL12A Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID16159
- Target nameIl12a
- Target descriptioninterleukin 12a
- Target synonymsIL-12p35, Il-12a, Ll12a, p35, interleukin-12 subunit alpha, CLMF p35, IL-12 p35 subunit, IL-12 subunit p35, cytotoxic lymphocyte maturation factor 35 kDa subunit, interleukin 12a p35 subunit, interleukin-12 p35 subunit
- HostRabbit
- IsotypeIgG
- Protein IDP43431
- Protein NameInterleukin-12 subunit alpha
- ReactivityMouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Yang L, Liu H, Zhang L, et al. Effect of IL-17 in the development of colon cancer in mice. Oncol Lett. 2016,12(6):4929-4936. doi: 10.3892/ol.2016.5329Read this paper
Datasheet
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