Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-12 alpha Polyclonal Antibody, Unconjugated (bs-0767R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-12 alpha Polyclonal Antibody, Unconjugated (bs-0767R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
IL12A Polyclonal Antibody
BS-0767R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIl12a
Overview
- SupplierBioss Antibodies
- Product NameIL-12 alpha Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID16159
- Target nameIl12a
- Target descriptioninterleukin 12a
- Target synonymsCLMF p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12; IL-12 p35 subunit; IL-12 subunit p35; Il-12a; IL-12p35; interleukin 12a p35 subunit; interleukin-12 p35 subunit; interleukin-12 subunit alpha; Ll12a; p3; p35
- HostRabbit
- IsotypeIgG
- Protein IDP43431
- Protein NameInterleukin-12 subunit alpha
- Storage Instruction-20°C
- UNSPSC12352203
References
- Effect of IL-17 in the development of colon cancer in mice. Yang L et al., 2016 Dec, Oncol LettRead more
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