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ProductsBack/IL-13 (A-9) Alexa Fluor(r) 594

IL-13 (A-9) Alexa Fluor(r) 594

SC-393365AF594
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse
TargetIl13
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    IL-13 (A-9) Alexa Fluor(r) 594
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 594
  • Gene ID16163
  • Target name
    Il13
  • Target description
    interleukin 13
  • Target synonyms
    Il-13, interleukin-13, T-cell activation protein P600
  • Host
    Mouse
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Il13 using anti-Il13 antibody (A00077-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Il13 antigen affinity purified polyclonal antibody (Catalog # A00077-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Il13 at approximately 19 kDa. The expected band size for Il13 is at 19 kDa.
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