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Lane 1: Mouse plasma lysates; Lane 2: Mouse hemocyte lysates probed with IL-1RA Polyclonal Antibody, Unconjugated (bs-2216R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse plasma lysates; Lane 2: Mouse hemocyte lysates probed with IL-1RA Polyclonal Antibody, Unconjugated (bs-2216R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse plasma lysates; Lane 2: Mouse hemocyte lysates probed with IL-1RA Polyclonal Antibody, Unconjugated (bs-2216R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

IL-1RA Polyclonal Antibody

BS-2216R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Sheep
TargetIl1rn
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Overview

  • Supplier
    Bioss
  • Product Name
    IL-1RA Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID16181
  • Target name
    Il1rn
  • Target description
    interleukin 1 receptor antagonist
  • Target synonyms
    F630041P17Rik, IL-1ra, interleukin-1 receptor antagonist protein, IL-1RN, IL1 inhibitor, IRAP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25085
  • Protein Name
    Interleukin-1 receptor antagonist protein
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL1RA/Il1rn using anti-IL1RA/Il1rn antibody (A00651-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RA/Il1rn antigen affinity purified polyclonal antibody (Catalog # A00651-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1RA/Il1rn at approximately 18KD. The expected band size for IL1RA/Il1rn is at 18KD.
Product group Antibodies
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ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetIl1rn
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TargetIl1rn
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