IL-31 Responsive Luciferase Reporter HEK293 Cell Line

BPS Bioscience

IL-31 Responsive Luciferase Reporter HEK293 Cell Line

23

Neutralisation/Blocking

Research Use Only

IL-31 Responsive Luciferase Reporter HEK293 Cell Line was engineered to express the human IL-31 receptor complex, composed of human IL-31 receptor alpha (hIL-31RA [NM_139017.7]) and human oncostatin M receptor beta (hOSMR [NM_003999.3]) separated by a self-cleaving P2A peptide. The cell line was generated by lentiviral transduction of STAT3 Luciferase Reporter HEK293 cells (cat#79800-P) which express firefly luciferase driven by STAT3 response elements located upstream of a minimal TATA promoter. IL-31 activity can be monitored by measuring luciferase activity. The functionality of this cell line was validated as responding to human IL-31, and by showing that IL-31-induced luciferase activity can be inhibited by anti-IL31 neutralizing antibodies. Figure 1: Illustration of the mechanism of IL-31 Responsive Luciferase Reporter HEK293 Cell Line. IL-31 initially binds to IL-31RA and then to OSMR to form a high affinity receptor complex. This binding leads to phosphorylation of STAT3 via the associated JAK1/2 pathways. After phosphorylation, STAT3 is translocated to the nucleus where it induces the transcription of firefly luciferase after binding to the response elements. Interested in screening and profiling inhibitors or blocking antibodies in a cell culture assay without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Liquid Nitrogen

12352207