IL-31RA: IL-31 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

BPS Bioscience

IL-31RA: IL-31 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

23

Research Use Only

Biotin

Interleukin-31 receptor subunit alpha

The IL-31RA: IL-31 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between IL-31RA (interleukin-31 receptor alpha subunit) and IL-31 protein for screening and profiling applications. This kit comes with enough purified IL-31RA (amino acids 20-519) and biotinylated IL-31 (amino acids 24-164(end)), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. IL-31RA: IL-31 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with IL-31RA protein. After coating and blocking, biotinylated IL-31 is added in an optimized assay buffer. Next, unbound biotinylated IL-31 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL-31 binding to IL-31RA. Need us to run inhibitor screens or profile your compounds that block the binding of IL31to IL31RA? Check out our Immunotherapy Biochemical Screening Services.

41116100

Human