IL-33 Responsive Luciferase Reporter Jurkat Cell Line

BPS Bioscience

IL-33 Responsive Luciferase Reporter Jurkat Cell Line

23

Research Use Only

The IL-33 Responsive Luciferase Reporter Jurkat Cell Line is a Jurkat cell line engineered to express both human IL33Ra (IL-33 receptor alpha) (NM_016232.5) and human IL1RAP (IL-1 Receptor Accessory Protein) (NM_002182.4) separated by a self-cleaving P2A peptide. The construct was delivered by lentiviral transduction of NF-kappaB Luciferase Reporter Jurkat Cells (#60651), which express a firefly luciferase reporter driven by NF-kappaB (nuclear factor Kappa B) response elements located upstream of the minimal TATA promoter. After activation by IL-33, the endogenous transcription factor NF-kappaB binds to the response elements, inducing transcription of the luciferase reporter gene. This cell line has been validated to respond to IL-33. Figure 1: Illustration of the mechanism of IL-33 Luciferase Reporter Jurkat Cell Line. IL-33 binds to its cognate receptor to activate downstream signal pathways that converge on transcription factor NF-kappaB, which translocates into the nucleus where it can activate the transcription of the Firefly luciferase reporter driven by NF-kappaB response elements present in the promoter. Interested in having us perform the screening and profiling of inhibitors or blocking antibodies? Visit our Immunotherapy Biochemical Screening Services.

Liquid Nitrogen

41116106

Human, Primate