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IL-6R Antibody (YA3720, Mouse)

HY-P84023
MedChem Express
TargetIL6R
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    IL-6R Antibody (YA3720, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3570
  • Target name
    IL6R
  • Target description
    interleukin 6 receptor
  • Target synonyms
    CD126; CD126 antigen; gp80; HIES5; IL-1Ra; IL-6 receptor subunit alpha; IL6Q; IL6QTL; IL-6R; IL-6R-1; IL6RA; IL-6RA; IL6RQ; interleukin-6 receptor subunit alpha; membrane glycoprotein 80
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    IL-6R Antibody (YA3720) is a mouse-derived and non-conjugated IgG1 antibody, targeting to CD126. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL6R using anti-IL6R antibody (A01425-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6R antigen affinity purified polyclonal antibody (Catalog # A01425-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6R at approximately 52KD. The expected band size for IL6R is at 52KD.
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