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IL1 beta antibody

GTX74034
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIl1b
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL1 beta antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. ELISA: 0.5-2microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID16176
  • Target name
    Il1b
  • Target description
    interleukin 1 beta
  • Target synonyms
    IL-1beta, Il-1b, interleukin-1 beta, IL-1 beta, interleukin 1b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10749
  • Protein Name
    Interleukin-1 beta
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Nomura M, Murad NF, Madhavan SS, et al. A ketogenic diet reduces age-induced chronic neuroinflammation in mice Running title: ketogenic diet and brain inflammaging. bioRxiv. 2023,:pii: 2023.12.01.569598. doi: 10.1101/2023.12.01.569598.
    Read this paper
  • Eeckhout E, Asaoka T, Van Gorp H, et al. The autoinflammation-associated NLRC4(V341A) mutation increases microbiota-independent IL-18 production but does not recapitulate human autoinflammatory symptoms in mice. Front Immunol. 2023,14:1272639. doi: 10.3389/fimmu.2023.1272639
    Read this paper
  • Ryu S, Spadaro O, Sidorov S, et al. Reduction of SPARC protects mice against NLRP3 inflammasome activation and obesity. J Clin Invest. 2023,133(19). doi: 10.1172/JCI169173
    Read this paper
  • Cui J, Li H, Ye D, et al. Inhibiting NINJ1-dependent plasma membrane rupture protects against inflammasome-induced blood coagulation and inflammation. bioRxiv. 2024,:pii: 2023.08.30.555561. doi: 10.1101/2023.08.30.555561.
    Read this paper
  • Pandeya A, Zhang Y, Cui J, et al. Inflammasome activation and pyroptosis mediate coagulopathy and inflammation in Salmonella systemic infection. Microbiol Res. 2023,275:127460. doi: 10.1016/j.micres.2023.127460
    Read this paper
  • Chen SH, Wu CC, Tseng WL, et al. Adipose-derived stem cells modulate neuroinflammation and improve functional recovery in chronic constriction injury of the rat sciatic nerve. Front Neurosci. 2023,17:1172740. doi: 10.3389/fnins.2023.1172740
    Read this paper
  • Yeh JL, Kuo CH, Shih PW, et al. Xanthine derivative KMUP-1 ameliorates retinopathy. Biomed Pharmacother. 2023,165:115109. doi: 10.1016/j.biopha.2023.115109
    Read this paper
  • Pourcelot M, da Silva Moraes RA, Lacour S, et al. Activation of Inflammasome during Bluetongue Virus Infection. Pathogens. 2023,12(6). doi: 10.3390/pathogens12060801
    Read this paper
  • Liu PY, Chen CY, Lin YL, et al. RNF128 regulates neutrophil infiltration and myeloperoxidase functions to prevent acute lung injury. Cell Death Dis. 2023,14(6):369. doi: 10.1038/s41419-023-05890-1
    Read this paper
  • Eeckhout E, Hamerlinck L, Jonckheere V, et al. Gasdermin D independent canonical inflammasome responses cooperate with caspase-8 to establish host defense against gastrointestinal Citrobacter rodentium infection. Cell Death Dis. 2023,14(4):282. doi: 10.1038/s41419-023-05801-4
    Read this paper

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Figure 1. Western blot analysis of IL1 beta using anti-IL1 beta antibody (A00101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7(-LPS) whole cell lysates, Lane 2: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody (Catalog # A00101-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 36 kDa. The expected band size for IL1 beta is at 31 kDa.
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