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ProductsBack/IL10 Rat Monoclonal Antibody [Clone ID: 12G8]

IL10 Rat Monoclonal Antibody [Clone ID: 12G8]

DDX0320P-100
OriGene
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIL10
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    IL10 Rat Monoclonal Antibody [Clone ID: 12G8]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    12G8
  • Gene ID3586
  • Target name
    IL10
  • Target description
    interleukin 10
  • Target synonyms
    CSIF, GVHDS, IL-10, IL10A, TGIF, interleukin-10, T-cell growth inhibitory factor, cytokine synthesis inhibitory factor
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP22301
  • Protein Name
    Interleukin-10
  • Scientific Description
    IL10 rat monoclonal antibody, clone 12G8
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (A00021-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysate, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse SP20 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
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