Sandwich ELISA analysis of human IL12 protein in duplicate at 600, 240, 96, 38.4, 15.4, 0 pg/ml (50 ul per lane) using GTX79487 as capture antibody (7 ug/ml) and GTX35255 as detection antibody (0.03125 ug/ml). The plate was washed then incubated with Streptavidin-HRP. Detection was performed with TMB substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
Sandwich ELISA analysis of human IL12 protein in duplicate at 600, 240, 96, 38.4, 15.4, 0 pg/ml (50 ul per lane) using GTX79487 as capture antibody (7 ug/ml) and GTX35255 as detection antibody (0.03125 ug/ml). The plate was washed then incubated with Streptavidin-HRP. Detection was performed with TMB substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
IL12 antibody [C8.6] (Biotin)
GTX35255
Overview
- SupplierGeneTex
- Product NameIL12 antibody [C8.6] (Biotin)
- Delivery Days Customer9
- Antibody SpecificityThis antibody is specific for natural IL-12 and recognizes the p70 heterodimer as well as the p40 subunit (monomers and homodimers). It will not cross-react with the p35 subunit.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDC8.6
- Concentration0.5 mg/ml
- ConjugateBiotin
- Gene ID3592
- Target nameIL12A
- Target descriptioninterleukin 12A
- Target synonymsCLMF; CLMF p35; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12, subunit p35; IL-12A; IL35 subunit; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; P35
- HostMouse
- IsotypeIgG1
- Protein IDP29459
- Protein NameInterleukin-12 subunit alpha
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203