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Western Blot analysis of COLO cells using IL-15Ralpha Polyclonal Antibody
Western Blot analysis of COLO cells using IL-15Ralpha Polyclonal Antibody
Western Blot analysis of COLO cells using IL-15Ralpha Polyclonal Antibody

IL15RA Antibody

CSB-PA011216
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetIL15RA
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL15RA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3601
  • Target name
    IL15RA
  • Target description
    interleukin 15 receptor subunit alpha
  • Target synonyms
    CD215; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13261
  • Protein Name
    Interleukin-15 receptor subunit alpha
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Paraformaldehyde-fixed, paraffin-embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL15RA) Polyclonal Antibody, Unconjugated (bs-2605R) at 1:200 overnight at 4°C, followed a conjugated secondary antibody and DAB staining.
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Figure 1. Western blot analysis of IL15RA using anti-IL15RA antibody (A03016-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL15RA antigen affinity purified polyclonal antibody (Catalog # A03016-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL15RA at approximately 28KD. The expected band size for IL15RA is at 28KD.
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