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Il18 Antibody

CSB-PA011608ZA01MO
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetIl18
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Overview

  • Supplier
    Cusabio
  • Product Name
    Il18 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID16173
  • Target name
    Il18
  • Target description
    interleukin 18
  • Target synonyms
    Igif, Il-18, interleukin-18, IFN-gamma-inducing factor, IL-1 gamma, interferon gamma-inducing factor, interleukin-1 gamma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP70380
  • Protein Name
    Interleukin-18
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL18 using anti-IL18 antibody (A00124-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # A00124-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.
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