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WB analysis of HepG2 cells using GTX47071 IL18RAP antibody at 2.5microg/ml.
WB analysis of HepG2 cells using GTX47071 IL18RAP antibody at 2.5microg/ml.
WB analysis of HepG2 cells using GTX47071 IL18RAP antibody at 2.5microg/ml.

IL18 Receptor beta antibody, N-term

GTX47071
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetIL18RAP
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL18 Receptor beta antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8807
  • Target name
    IL18RAP
  • Target description
    interleukin 18 receptor accessory protein
  • Target synonyms
    ACPL; CD218 antigen-like family member B; CD218b; CDw218b; cluster of differentiation w218b; IL-18 receptor accessory protein; IL-18 receptor beta; IL-18RAcP; IL18RB; IL-18Rbeta; IL-18R-beta; IL-1R7; IL-1R-7; IL-1RAcPL; interleukin-1 receptor 7; interleukin-18 receptor accessory protein; interleukin-18 receptor beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95256
  • Protein Name
    Interleukin-18 receptor accessory protein
  • Scientific Description
    The protein encoded by this gene is an accessory subunit of the heterodimeric receptor for interleukin 18 (IL18), a proinflammatory cytokine involved in inducing cell-mediated immunity. This protein enhances the IL18-binding activity of the IL18 receptor and plays a role in signaling by IL18. Mutations in this gene are associated with Crohns disease and inflammatory bowel disease, and susceptibility to celiac disease and leprosy. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL-18R Beta/IL-18RAP using anti-IL-18R Beta/IL-18RAP antibody (A06457-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-18R Beta/IL-18RAP antigen affinity purified polyclonal antibody (Catalog # A06457-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-18R Beta/IL-18RAP at approximately 72KD. The expected band size for IL-18R Beta/IL-18RAP is at 72KD.
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