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Il18bp Antibody Pair

CSB-EAP16769
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityCanine, Mouse, Porcine
TargetIl18bp
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Overview

  • Supplier
    Cusabio
  • Product Name
    Il18bp Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID16068
  • Target name
    Il18bp
  • Target description
    interleukin 18 binding protein
  • Target synonyms
    Igi; Igifbp; IL-1; IL-18BP; interferon gamma-inducing factor-binding protein; interleukin-18-binding protein; MC54; MC54L
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Z0M9
  • Protein Name
    Interleukin-18-binding protein
  • Reactivity
    Canine, Mouse, Porcine
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of IL18 binding protein using anti-IL18 binding protein antibody (A07261-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse heart tissue lysate, Lane 2: mouse brain tissue lysate, Lane 3: rat heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 binding protein antigen affinity purified polyclonal antibody (Catalog # A07261-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 binding protein at approximately 43KD. The expected band size for IL18 binding protein is at 21KD.
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