Western blot using GeneTexs Protein-A Purified anti-bovine IL-1F5 antibody shows detection of recombinant bovine IL-1F5 at 17.1 kDa (arrowhead) raised in yeast. The identity of the band at 36 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA, and probed with 1 microg/mL primary antibody overnight at 4oC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
Western blot using GeneTexs Protein-A Purified anti-bovine IL-1F5 antibody shows detection of recombinant bovine IL-1F5 at 17.1 kDa (arrowhead) raised in yeast. The identity of the band at 36 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA, and probed with 1 microg/mL primary antibody overnight at 4oC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
IL1F5 antibody
GTX48763
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine
TargetIL36RN
Overview
- SupplierGeneTex
- Product NameIL1F5 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1 microg/mL. ELISA: 1:8000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID518514
- Target nameIL36RN
- Target descriptioninterleukin 36 receptor antagonist
- Target synonymsIL1F5; interleukin 1 family, member 5 (delta); interleukin-36 receptor antagonist protein
- HostRabbit
- IsotypeIgG
- Scientific DescriptionInterleukin-1F5 is a recently discovered cytokine which was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6) in humans. There is very little published regarding the bovine IL1F5 protein. In the human, this gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Expression of the gene appears to be restricted to various epithelial cell populations. Costelloe et al (2008) have identified SIGIRR [single immunoglobulin domain-containing IL1R-related protein] as a receptor for human IL1F5.
- ReactivityBovine
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203