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IL1RL1 Antibody

LS-C814573
LifeSpan BioSciences
ApplicationsELISA, Other Application
Product group Antibodies
TargetIL1RL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IL1RL1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ELISA, Other Application
  • Applications Supplier
    ELISA, LateralFlow Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9173
  • Target name
    IL1RL1
  • Target description
    interleukin 1 receptor like 1
  • Target synonyms
    DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1, interleukin-1 receptor-like 1, growth stimulation-expressed, homolog of mouse growth stimulation-expressed, interleukin 1 receptor-related protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ST2/IL1RL1 using anti-ST2/IL1RL1 antibody (A00492-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST2/IL1RL1 antigen affinity purified polyclonal antibody (Catalog # A00492-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST2/IL1RL1 at approximately 70 kDa. The expected band size for ST2/IL1RL1 is at 63 kDa.
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Detection of endogenous human ST2 with anti-ST2 (human), pAb (AG-25A-0058). Method: THP1 cells were stained with anti-ST2 (human), pAb (1:100 in PBS + 2% FCS) (Figure B) or with the secondary antibody alone (Figure A) for 1h at 4°C. The staining wa
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