![ELISA analysis of antigen using GTX60792 IL2 Receptor alpha antibody [1B5D12]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng ELISA analysis of antigen using GTX60792 IL2 Receptor alpha antibody [1B5D12]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60792/GTX60792_20170912_ELISA_w_23061123_556.webp)
ELISA analysis of antigen using GTX60792 IL2 Receptor alpha antibody [1B5D12]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
IL2 Receptor alpha antibody [1B5D12]
GTX60792
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIL2RA
Overview
- SupplierGeneTex
- Product NameIL2 Receptor alpha antibody [1B5D12]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1B5D12
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3559
- Target nameIL2RA
- Target descriptioninterleukin 2 receptor subunit alpha
- Target synonymsCD25, IDDM10, IL2R, IMD41, TCGFR, p55, interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2R subunit alpha, TAC antigen, interleukin 2 receptor, alpha
- HostMouse
- IsotypeIgG1
- Protein IDP01589
- Protein NameInterleukin-2 receptor subunit alpha
- Scientific DescriptionThe interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![ICC/IF analysis of HeLa cells using GTX60792 IL2 Receptor alpha antibody [1B5D12]. Green : IL2 Receptor alpha Blue: DRAQ5 fluorescent DNA dye ICC/IF analysis of HeLa cells using GTX60792 IL2 Receptor alpha antibody [1B5D12]. Green : IL2 Receptor alpha Blue: DRAQ5 fluorescent DNA dye](https://www.genetex.com/upload/website/prouct_img/normal/GTX60792/GTX60792_20170912_ICCIF_w_23061123_843.webp)
![IHC-P analysis of rectum cancer tissue using GTX60792 IL2 Receptor alpha antibody [1B5D12]. IHC-P analysis of rectum cancer tissue using GTX60792 IL2 Receptor alpha antibody [1B5D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60792/GTX60792_20170912_IHC-P_1_w_23061123_978.webp)
![IHC-P analysis of bladder cancer tissue using GTX60792 IL2 Receptor alpha antibody [1B5D12]. IHC-P analysis of bladder cancer tissue using GTX60792 IL2 Receptor alpha antibody [1B5D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60792/GTX60792_20170912_IHC-P_w_23061123_325.webp)
![WB analysis of HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH3T3 (9) cell lysate using GTX60792 IL2 Receptor alpha antibody [1B5D12]. WB analysis of HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH3T3 (9) cell lysate using GTX60792 IL2 Receptor alpha antibody [1B5D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60792/GTX60792_20170912_WB_1_w_23061123_386.webp)
![WB analysis of HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate using GTX60792 IL2 Receptor alpha antibody [1B5D12]. WB analysis of HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate using GTX60792 IL2 Receptor alpha antibody [1B5D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60792/GTX60792_20170912_WB_w_23061123_782.webp)

![IHC-P analysis of human tonsil tissue using GTX18115 IL2 Receptor alpha antibody [IL2RA/2395].](https://www.genetex.com/upload/website/prouct_img/normal/GTX18115/GTX18115_20200115_IHC-P_1294_w_23060620_923.webp)
![FACS analysis of human PBMC cells using GTX18275 IL2 Receptor alpha antibody [MEM-181] (FITC). The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days. Panel A, C : staining with GTX18275 Panel B, D : staining with a positive control anti-CD25 monoclonal antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX18275/GTX18275_20191025_AP_006_143_w_23060620_970.webp)
![FACS analysis of human peripheral blood lymphocytes using GTX01471-07 IL2 Receptor alpha antibody [BC96] (APC). Right panel : co-stained with IL2 Receptor alpha antibody [BC96] (APC) and Human CD4 antibody (FITC) Left panel : co-stained with isotype control and Human CD4 antibody (FITC) antibody amount : 0.125 microg (5 microl)](https://www.genetex.com/upload/website/prouct_img/normal/GTX01471-07/GTX01471-07_20200428_FACS13_w_23053121_178.webp)
![FACS analysis of human peripheral blood lymphocytes using GTX01471-08 IL2 Receptor alpha antibody [BC96] (PE). Right panel : primary antibody Left panel : isotype control antibody amount : 0.06 microg (5 microl)](https://www.genetex.com/upload/website/prouct_img/normal/GTX01471-08/GTX01471-08_20200428_FACS103_w_23053121_163.webp)
![FACS analysis of human peripheral blood lymphocytes using GTX01471-10 IL2 Receptor alpha antibody [BC96] (PE-Cy7). Right panel : co-stained with IL2 Receptor alpha antibody [BC96] (PE-Cy7) and Human CD4 antibody (FITC) Left panel : co-stained with isotype control and Human CD4 antibody (FITC) antibody amount : 0.25 microg (5 microl)](https://www.genetex.com/upload/website/prouct_img/normal/GTX01471-10/GTX01471-10_20200428_FACS141_w_23053121_777.webp)