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IL22 antibody detects IL22 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. IL22 antibody (GTX109659) dilution: 1:250. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
IL22 antibody detects IL22 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. IL22 antibody (GTX109659) dilution: 1:250. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
IL22 antibody detects IL22 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. IL22 antibody (GTX109659) dilution: 1:250. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

IL22 antibody

GTX109659
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIL22
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL22 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID50616
  • Target name
    IL22
  • Target description
    interleukin 22
  • Target synonyms
    IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18, interleukin-22, IL-10-related T-cell-derived inducible factor, cytokine Zcyto18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZX6
  • Protein Name
    Interleukin-22
  • Scientific Description
    Cytokine that contributes to the inflammatory response in vivo.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wu Y, Tan L, Shi L, et al. Interleukin-22 is elevated in the atrium and plasma of patients with atrial fibrillation and increases collagen synthesis in transforming growth factor-β1-treated cardiac fibroblasts via the JNK pathway. Exp Ther Med. 2020,20(2):1012-1020. doi: 10.3892/etm.2020.8778
    Read this paper
  • Ji YW, Mittal SK, Hwang HS, et al. Lacrimal gland-derived IL-22 regulates IL-17-mediated ocular mucosal inflammation. Mucosal Immunol. 2017,10(5):1202-1210. doi: 10.1038/mi.2016.119
    Read this paper

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Figure 1. Western blot analysis of IL22 using anti-IL22 antibody (A00963-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL22 antigen affinity purified polyclonal antibody (Catalog # A00963-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL22 at approximately 24 kDa. The expected band size for IL22 is at 20 kDa.
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