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Il23R Polyclonal Antibody

CAC07795
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIL23R
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Overview

  • Supplier
    Biomatik
  • Product Name
    Il23R Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID149233
  • Target name
    IL23R
  • Target description
    interleukin 23 receptor
  • Target synonyms
    PSORS7, interleukin-23 receptor, IL-23 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VWK5
  • Protein Name
    Interleukin-23 receptor
  • Scientific Description
    The Il23R Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: HL60 cell lysates probed with IL-23R Polyclonal Antibody, Unconjugated (bs-1460R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
IL-23R Polyclonal AntibodyBS-1460R
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Figure 1. Western blot analysis of IL23 Receptor/IL23R using anti-IL23 Receptor/IL23R antibody (A00607-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 Receptor/IL23R antigen affinity purified polyclonal antibody (Catalog # A00607-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 Receptor/IL23R at approximately 72KD. The expected band size for IL23 Receptor/IL23R is at 72KD.
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Anti-IL23 Receptor/IL23R Antibody Picoband(r)A00607-3-CARRIER-FREE
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WB analysis of HeLa cell lysate using GTX54301 IL23 Receptor antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
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The image on the left is immunohistochemistry of paraffin-embedded Human rectal cancer tissue using CSB-PA273292(IL23R Antibody) at dilution 1/10, on the right is treated with fusion protein. (Original magnification: x200)
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