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ProductsBack/IL3 Mouse Monoclonal Antibody [Clone ID: OTI3B11]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL-3 (Cat# RC210109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-3(Cat# TA500182). Positive lysates [LY424621] (100ug) and [LC424621] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL-3 (Cat# RC210109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-3(Cat# TA500182). Positive lysates [LY424621] (100ug) and [LC424621] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL-3 (Cat# RC210109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-3(Cat# TA500182). Positive lysates [LY424621] (100ug) and [LC424621] (20ug) can be purchased separately from OriGene.

IL3 Mouse Monoclonal Antibody [Clone ID: OTI3B11]

CF500182
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL3
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    IL3 Mouse Monoclonal Antibody [Clone ID: OTI3B11]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3B11
  • Gene ID3562
  • Target name
    IL3
  • Target description
    interleukin 3
  • Target synonyms
    IL-3, MCGF, MULTI-CSF, interleukin-3, P-cell stimulating factor, colony-stimulating factor, multiple, hematopoietic growth factor, mast-cell growth factor, multilineage-colony-stimulating factor, multipotential colony-stimulating factor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08700
  • Protein Name
    Interleukin-3
  • Scientific Description
    Carrier-free (BSA/glycerol-free) IL-3 mouse monoclonal antibody, clone OTI3B11 (formerly 3B11)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure. Western blot analysis of IL-3 using anti-IL-3 antibody (RP1008). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Human IL-3 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-3 antigen affinity purified polyclonal antibody (Catalog # RP1008) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-3 at approximately 15KD. The expected band size for IL-3 is at 15KD.
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