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Western Blot Positive WB detected in: PC-3 whole cell lysate All lanes: IL34 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa
Western Blot Positive WB detected in: PC-3 whole cell lysate All lanes: IL34 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa
Western Blot Positive WB detected in: PC-3 whole cell lysate All lanes: IL34 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa

IL34 Antibody

CSB-PA751146LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL34
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL34 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID146433
  • Target name
    IL34
  • Target description
    interleukin 34
  • Target synonyms
    C16orf77; IL-34; interleukin-34
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZMJ4
  • Protein Name
    Interleukin-34
  • Scientific Description
    Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL34 using anti-IL34 antibody (A06903-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whle cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human HEK293 whole cell lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL34 antigen affinity purified polyclonal antibody (Catalog # A06903-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL34 at approximately 39 kDa. The expected band size for IL34 is at 27 kDa.
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