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ProductsBack/IL36 alpha (IL36A) Mouse Monoclonal Antibody [Clone ID: OTI3A2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL1F6 (Cat# RC219328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6(Cat# TA501459). Positive lysates [LY415288] (100ug) and [LC415288] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL1F6 (Cat# RC219328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6(Cat# TA501459). Positive lysates [LY415288] (100ug) and [LC415288] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL1F6 (Cat# RC219328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6(Cat# TA501459). Positive lysates [LY415288] (100ug) and [LC415288] (20ug) can be purchased separately from OriGene.

IL36 alpha (IL36A) Mouse Monoclonal Antibody [Clone ID: OTI3A2]

CF501459
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetIL36A
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    IL36 alpha (IL36A) Mouse Monoclonal Antibody [Clone ID: OTI3A2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3A2
  • Gene ID27179
  • Target name
    IL36A
  • Target description
    interleukin 36 alpha
  • Target synonyms
    FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6, interleukin-36 alpha, FIL1 epsilon, IL-1 epsilon, IL-1F6 (FIL-1-epsilon), interleukin 1 family, member 6 (epsilon), interleukin-1 epsilon, interleukin-1 family member 6
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9UHA7
  • Protein Name
    Interleukin-36 alpha
  • Scientific Description
    Carrier-free (BSA/glycerol-free) IL1F6 mouse monoclonal antibody, clone OTI3A2 (formerly 3A2)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of IL36 alpha using anti-IL36 alpha antibody (A09802-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL36 alpha antigen affinity purified polyclonal antibody (Catalog # A09802-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL36 alpha at approximately 25KD. The expected band size for IL36 alpha is at 17KD.
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