FACS analysis of human peripheral blood lymphocytes (with PMA and Ionomycin stimulation) using GTX01492-07 IL4 antibody [MP4-25D2] (APC). Right panel : co-stained with IL4 antibody [MP4-25D2] (APC) and Human CD3 antibody (PE) Left panel : co-stained with isotype control and Human CD3 antibody (PE) antibody amount : 0.0375 microg (5 microl)
FACS analysis of human peripheral blood lymphocytes (with PMA and Ionomycin stimulation) using GTX01492-07 IL4 antibody [MP4-25D2] (APC). Right panel : co-stained with IL4 antibody [MP4-25D2] (APC) and Human CD3 antibody (PE) Left panel : co-stained with isotype control and Human CD3 antibody (PE) antibody amount : 0.0375 microg (5 microl)
IL4 antibody [MP4-25D2] (APC)
GTX01492-07
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman, Primate
TargetIL4
Overview
- SupplierGeneTex
- Product NameIL4 antibody [MP4-25D2] (APC)
- Delivery Days Customer9
- Application Supplier NoteFACS: 0.0375 microg (5 microl) for 105-108 cells in 100 microl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDMP4-25D2
- Concentration0.0075 mg/ml
- ConjugateAPC (Allophycocyanin)
- FormulationLiquid
- Gene ID3565
- Target nameIL4
- Target descriptioninterleukin 4
- Target synonymsB cell growth factor 1; B_cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL-4; interleukin 4 variant 2; interleukin-4; lymphocyte stimulatory factor 1; pitrakinra
- HostRat
- IsotypeIgG1
- Protein IDP05112
- Protein NameInterleukin-4
- Scientific DescriptionThe protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Primate
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. Prussin C et al., 1995 Dec 15, J Immunol MethodsRead more
- Interleukin-4 and interleukin-5 are rarely co-expressed by human T cells. Jung T et al., 1995 Aug, Eur J ImmunolRead more
- Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4. Chretien I et al., 1989 Feb 8, J Immunol MethodsRead more
- Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Abrams JS et al., 1992 Jun, Immunol RevRead more