More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IL4 antibody [MP4-25D2] (APC)
FACS analysis of human peripheral blood lymphocytes (with PMA and Ionomycin stimulation) using GTX01492-07 IL4 antibody [MP4-25D2] (APC). Right panel : co-stained with IL4 antibody [MP4-25D2] (APC) and Human CD3 antibody (PE) Left panel : co-stained with isotype control and Human CD3 antibody (PE) antibody amount : 0.0375 microg (5 microl)
FACS analysis of human peripheral blood lymphocytes (with PMA and Ionomycin stimulation) using GTX01492-07 IL4 antibody [MP4-25D2] (APC). Right panel : co-stained with IL4 antibody [MP4-25D2] (APC) and Human CD3 antibody (PE) Left panel : co-stained with isotype control and Human CD3 antibody (PE) antibody amount : 0.0375 microg (5 microl)
FACS analysis of human peripheral blood lymphocytes (with PMA and Ionomycin stimulation) using GTX01492-07 IL4 antibody [MP4-25D2] (APC). Right panel : co-stained with IL4 antibody [MP4-25D2] (APC) and Human CD3 antibody (PE) Left panel : co-stained with isotype control and Human CD3 antibody (PE) antibody amount : 0.0375 microg (5 microl)

IL4 antibody [MP4-25D2] (APC)

GTX01492-07
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetIL4
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    IL4 antibody [MP4-25D2] (APC)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: 0.0375 microg (5 microl) for 105-108 cells in 100 microl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MP4-25D2
  • Concentration
    0.0075 mg/ml
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID3565
  • Target name
    IL4
  • Target description
    interleukin 4
  • Target synonyms
    BCGF-1, BCGF1, BSF-1, BSF1, IL-4, interleukin-4, B cell growth factor 1, B_cell stimulatory factor 1, binetrakin, lymphocyte stimulatory factor 1, pitrakinra
  • Host
    Rat
  • Isotype
    IgG1
  • Protein IDP05112
  • Protein Name
    Interleukin-4
  • Scientific Description
    The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995,188(1):117-28.
    Read this paper
  • Jung T, Schauer U, Rieger C, et al. Interleukin-4 and interleukin-5 are rarely co-expressed by human T cells. Eur J Immunol. 1995,25(8):2413-6.
    Read this paper
  • Chrétien I, Van Kimmenade A, Pearce MK, et al. Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4. J Immunol Methods. 1989,117(1):67-81.
    Read this paper
  • Abrams JS, Roncarolo MG, Yssel H, et al. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992,127:5-24.
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of Raji Jurkat cells using IL-4 Polyclonal Antibody
Product group Antibodies
Cusabio
IL4 AntibodyCSB-PA004908
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIL4
  • SizePrice
WB analysis of 1 ug of human recombiannt IL-4 protein in non-reducing sample buffer using GTX15758 IL4 antibody [E10021]. Dilution : 1:1000
Product group Antibodies
GeneTex
IL4 antibody [E10021]GTX15758
ApplicationsWestern Blot, ELISA
TargetIL4
  • SizePrice
WB analysis of 1 ug of human recombiannt IL-4 protein in non-reducing sample buffer using GTX15759 IL4 antibody [25D2]. Dilution : 1:1000
Product group Antibodies
GeneTex
IL4 antibody [25D2] (Biotin)GTX15759
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
TargetIL4
  • SizePrice
Product group Antibodies
GeneTex
IL4 antibodyGTX10755
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking
TargetIL4
  • SizePrice
WB analysis of various samples using GTX66813 IL4 antibody. Lane1 : Jurkat whole cell lysate(40ug) Lane2 : The testis tissue lysate of Mouse(40ug) Lane3 : The testis tissue lysate of Rat(40ug) Lane4 : The thymus tissue lysate of Rat(40ug) Lane5 : THP-1 whole cell lysate(40ug) Dilution : 1:500
Product group Antibodies
GeneTex
IL4 antibodyGTX66813
ApplicationsWestern Blot
TargetIL4
  • SizePrice
Product group Antibodies
MedChem Express
IL-4 Antibody (YA343, Rabbit)HY-P80722
TargetIL4
  • SizePrice
Product group Antibodies
GeneTex
IL4 antibody [4Z33]GTX52858
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
TargetIL4
  • SizePrice
Product group Antibodies
GeneTex
IL4 antibody [11-60]GTX10107
ApplicationsELISA
TargetIL4
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-IL-4 Recombinant Monoclonal Antibody [BLR298L]A700-298
ApplicationsImmunoPrecipitation, Western Blot
TargetIL4
  • SizePrice
Figure 1. Western blot analysis of IL4 using anti-IL4 antibody (A00230). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL4 antigen affinity purified polyclonal antibody (Catalog # A00230) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL4 at approximately 20KD. The expected band size for IL4 is at 17KD.
Product group Antibodies
Boster Bio
Anti-IL4 Antibody Picoband(r)A00230-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetIL4
  • SizePrice