Tissue/cell: A431 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with IL4R Polyclonal Antibody, Unconjugated (bs-2458R) at 1:100 for 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody with FITC conjugation (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue) was used to stain the cell nuclei.
Tissue/cell: A431 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with IL4R Polyclonal Antibody, Unconjugated (bs-2458R) at 1:100 for 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody with FITC conjugation (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue) was used to stain the cell nuclei.
IL-4R Polyclonal Antibody
BS-2458R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIL4R
Overview
- SupplierBioss
- Product NameIL-4R Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID3566
- Target nameIL4R
- Target descriptioninterleukin 4 receptor
- Target synonymsCD124, IL-4RA, IL4RA, interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, interleukin 13 receptor, interleukin-4 receptor alpha chain
- HostRabbit
- IsotypeIgG
- Protein IDP24394
- Protein NameInterleukin-4 receptor subunit alpha
- Storage Instruction-20°C
- UNSPSC12352203
References
- Danelli L, Frossi B, Gri G, et al. Mast cells boost myeloid-derived suppressor cell activity and contribute to the development of tumor-favoring microenvironment. Cancer Immunol Res. 2015,3(1):85-95. doi: 10.1158/2326-6066.CIR-14-0102Read this paper
Datasheet
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