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Tissue/cell: A431 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with IL4R Polyclonal Antibody, Unconjugated (bs-2458R) at 1:100 for 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody with FITC conjugation (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue) was used to stain the cell nuclei.
Tissue/cell: A431 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with IL4R Polyclonal Antibody, Unconjugated (bs-2458R) at 1:100 for 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody with FITC conjugation (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue) was used to stain the cell nuclei.
Tissue/cell: A431 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with IL4R Polyclonal Antibody, Unconjugated (bs-2458R) at 1:100 for 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody with FITC conjugation (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue) was used to stain the cell nuclei.

IL-4R Polyclonal Antibody

BS-2458R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIL4R
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    IL4R Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3566
  • Target name
    IL4R
  • Target description
    interleukin 4 receptor
  • Target synonyms
    CD124; IL-4 receptor subunit alpha; IL4R nirs variant 1; IL4RA; IL-4RA; interleukin 13 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24394
  • Protein Name
    Interleukin-4 receptor subunit alpha
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Mast cells boost myeloid-derived suppressor cell activity and contribute to the development of tumor-favoring microenvironment. Danelli L et al., 2015 Jan, Cancer Immunol Res
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