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IL6 antibody

GTX110527
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetIL6
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.72 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3569
  • Target name
    IL6
  • Target description
    interleukin 6
  • Target synonyms
    B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor; HGF; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL-6; interferon beta-2; interleukin BSF-2; interleukin-6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05231
  • Protein Name
    Interleukin-6
  • Scientific Description
    This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Tumor suppressor p53 mediates interleukin-6 expression to enable cancer cell evasion of genotoxic stress.
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  • 4-PBA inhibits endoplasmic reticulum stress to improve autophagic flux in the treatment of protamine/lipopolysaccharide-induced interstitial cystitis in rats.
    Read more
  • Anti-Inflammatory Effects of Adiponectin Receptor Agonist AdipoRon against Intervertebral Disc Degeneration.
    Read more
  • Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-beta/TbetaRs signaling pathway.
    Read more
  • Novel long non-coding RNAs associated with inflammation and macrophage activation in human.
    Read more
  • p16(INK4a) Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin.
    Read more
  • Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
    Read more
  • Characteristics of pre-sensitization-related acute antibody-mediated rejection in a rat model of orthotopic liver transplantation.
    Read more
  • Cross-Linked Hyaluronate and Corticosteroid Combination Ameliorate the Rat Experimental Tendinopathy through Anti-Senescent and -Apoptotic Effects.
    Read more
  • IFIT2-depleted metastatic oral squamous cell carcinoma cells induce muscle atrophy and cancer cachexia in mice. Lai KC et al., 2022 Apr, J Cachexia Sarcopenia Muscle
    Read more

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Figure 1. Western blot analysis of IL-6 using anti-IL-6 antibody (RP1012). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL6 protein 10 ng, Lane 2: recombinant human IL6 protein 5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-6 antigen affinity purified polyclonal antibody (Catalog # RP1012) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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