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ELISA analysis of rat IL-6 recombinant protein(0.2 - 0.4 ng/well) using GTX29770 IL6 antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Rat IL-6 as a detection antibody.
ELISA analysis of rat IL-6 recombinant protein(0.2 - 0.4 ng/well) using GTX29770 IL6 antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Rat IL-6 as a detection antibody.
ELISA analysis of rat IL-6 recombinant protein(0.2 - 0.4 ng/well) using GTX29770 IL6 antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Rat IL-6 as a detection antibody.

IL6 antibody

GTX29770
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
DownloadDatasheet
Product group Antibodies
TargetIl6
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1 - 0.2 microg/mL. ELISA: 0.5 microg/mL. Neutralizing/Inhibition: 0.3 - 0.5 microg/mL. Sandwich ELISA: 0.5 - 2.0 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID24498
  • Target name
    Il6
  • Target description
    interleukin 6
  • Target synonyms
    Ifnb2; IL-6; ILg6; Interleukin 6 (interferon, beta 2); interleukin-6
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP20607
  • Protein Name
    Interleukin-6
  • Scientific Description
    Interleukin-6 is a potent lymphoid cell growth factor that affects B-lymphocytes, T lymphocytes, and hybridoma cells. It will also affect cytotoxic T cells in combination with other factors such as IL-2 and gamma-interferon.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL6 using anti-IL6 antibody (A00102-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse Ana-1 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates, Lane 5: mouse EL-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # A00102-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.
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