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ProductsBack/IL7 antibody [4J64]

IL7 antibody [4J64]

GTX53368
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetIl7
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL7 antibody [4J64]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:100 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4J64
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID16196
  • Target name
    Il7
  • Target description
    interleukin 7
  • Target synonyms
    A630026I06Rik, Il-7, hlb368, interleukin-7
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDP10168
  • Protein Name
    Interleukin-7
  • Scientific Description
    The protein encoded by this gene is a hematopoietic growth factor important for B and T cell development. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL7 using anti-IL7 antibody (PB9871). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: NIH3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody (Catalog # PB9871) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 20 kDa. The expected band size for IL7 is at 18 kDa.
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