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Western blot analysis of extracts of HepG2 cell lines, using IL7 antibody.
Western blot analysis of extracts of HepG2 cell lines, using IL7 antibody.
Western blot analysis of extracts of HepG2 cell lines, using IL7 antibody.

IL7 Antibody

CSB-PA011669KA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIL7
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3574
  • Target name
    IL7
  • Target description
    interleukin 7
  • Target synonyms
    IL-7; interleukin-7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13232
  • Protein Name
    Interleukin-7
  • Scientific Description
    IL-7 plays a key role in lymphopoiesis and lymphoid homeostasis. Stromal and epithelial cells within the bone marrow and thymus produce IL-7. The primary targets of IL-7 are T cells, B cells, and dendritic cells. IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling. While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear. In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of naive and memory alphabeta T cells, as well as gammadelta T cells. The IL-7 receptor is a heterodimer of the common gamma chain, gammac, and the IL-7-specific IL-7Ralpha. IL-7 activates PI3K/Akt, Jak1/2, and Stat1, 3, and 5.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL7 using anti-IL7 antibody (PB9521). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: K562 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody (Catalog # PB9521) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 75 kDa. The expected band size for IL7 is at 20 kDa.
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