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ProductsBack/IL9 antibody (Biotin)
Western Blot of Rabbit anti-Human IL-9 Biotin Conjugated Antibody. Load: 50 ng Human IL-9 per lane. Primary antibody: Human IL-9 primary antibody at 1:1,000 overnight at 4oC. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 14 kDa for Human IL-9. Other band(s): None.
Western Blot of Rabbit anti-Human IL-9 Biotin Conjugated Antibody. Load: 50 ng Human IL-9 per lane. Primary antibody: Human IL-9 primary antibody at 1:1,000 overnight at 4oC. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 14 kDa for Human IL-9. Other band(s): None.
Western Blot of Rabbit anti-Human IL-9 Biotin Conjugated Antibody. Load: 50 ng Human IL-9 per lane. Primary antibody: Human IL-9 primary antibody at 1:1,000 overnight at 4oC. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 14 kDa for Human IL-9. Other band(s): None.

IL9 antibody (Biotin)

GTX48670
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIL9
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL9 antibody (Biotin)
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000-1:10000. ELISA: 1:20000-1:100000. IHC: 1:1:1000-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Gene ID3578
  • Target name
    IL9
  • Target description
    interleukin 9
  • Target synonyms
    HP40, IL-9, P40, interleukin-9, T-cell growth factor p40, cytokine P40, homolog of mouse T cell and mast cell growth factor 40, p40 T-cell and mast cell growth factor, p40 cytokine
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15248
  • Protein Name
    Interleukin-9
  • Scientific Description
    IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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GeneTexs anti-Human IL-9 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-9. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
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