Flow-cytometry using the Anti-IL-9 antibody MH9A4 (TA386284). Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (clone 4-4-20; isotype control, black line) or the rabbit IgG version of MH9A4 (TA386284, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.
Flow-cytometry using the Anti-IL-9 antibody MH9A4 (TA386284). Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (clone 4-4-20; isotype control, black line) or the rabbit IgG version of MH9A4 (TA386284, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.
IL9 Rabbit Monoclonal Antibody [Clone ID: MH9A4]
TA386284
Overview
- SupplierOriGene
- Product NameIL9 Rabbit Monoclonal Antibody [Clone ID: MH9A4]
- Delivery Days Customer14
- ApplicationsFlow Cytometry, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDMH9A4
- Gene ID3578
- Target nameIL9
- Target descriptioninterleukin 9
- Target synonymscytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL-9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor p40
- HostRabbit
- IsotypeIgG
- Scientific DescriptionRecombinant Anti-IL-9 (Clone MH9A4)
- ReactivityHuman
- UNSPSC12352203