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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ILDR1 antibody at 2.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63, 58, 31, 24, 53, 60 kDa Observed band size: 63 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ILDR1 antibody at 2.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63, 58, 31, 24, 53, 60 kDa Observed band size: 63 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ILDR1 antibody at 2.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63, 58, 31, 24, 53, 60 kDa Observed band size: 63 kDa

ILDR1 Antibody

CSB-PA768201LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetILDR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ILDR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID286676
  • Target name
    ILDR1
  • Target description
    immunoglobulin like domain containing receptor 1
  • Target synonyms
    DFNB42, ILDR1alpha, ILDR1alpha', ILDR1beta, immunoglobulin-like domain-containing receptor 1, angulin-2, immunoglobulin-like domain-containing receptor 1 alpha, immunoglobulin-like domain-containing receptor 1 beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86SU0
  • Protein Name
    Immunoglobulin-like domain-containing receptor 1
  • Scientific Description
    Putative membrane receptor.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ILDR1 using anti-ILDR1 antibody (A09114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILDR1 antigen affinity purified polyclonal antibody (Catalog # A09114-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ILDR1 at approximately 63 kDa. The expected band size for ILDR1 is at 63 kDa.
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